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KMID : 0364819910290010001
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Korean Journal of Microbiology
1991 Volume.29 No. 1 p.1 ~ p.7
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Cloning and Expression of Human Immunodeficieny Virus-1 Epitopes in Escherichia coli
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Yoo Hyang-Sook
Jang Won-Hee
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Abstract
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1
Human immunodeficiency virus type 1 (HIV-1) causes a deadly infectious disease, Acquired Immunodeficiency Syndrome (AIDS). As a first step to develop a reliable and fast diagnostic procedure for HIV-1 infection, we cloned various immunodominant epitopes of H1V1 in bacterial expression vectors containing tac or trp promoter. While the protein level of direct expression of gpl6O was low, trp E fused gpl20, gp4I and p17-p24 were produced at high levels (15-30% of total bacterial proteins) in E. coli. Since gp120 and gp4l contain relatively conserved regions which can react with antibodies in the plasma from most of HIV-1 infected individuals, these expression clones were used for large preparations of HIV-1 antigens.
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KEYWORD
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